wild type wt skmel28 cells Search Results


melanoma cell lines skmel 28  (ATCC)
99
ATCC melanoma cell lines skmel 28
Melanoma Cell Lines Skmel 28, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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melanoma cell lines skmel 28 - by Bioz Stars, 2026-07
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cell culture skmel28 cells  (CLS Cell Lines Service GmbH)
94
CLS Cell Lines Service GmbH cell culture skmel28 cells
Cell Culture Skmel28 Cells, supplied by CLS Cell Lines Service GmbH, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pmc05066145__ml6b00340_si_001-140-0-7?v=CLS+Cell+Lines+Service+GmbH
Average 94 stars, based on 1 article reviews
cell culture skmel28 cells - by Bioz Stars, 2026-07
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transfection human melanoma lines  (ATCC)
96
ATCC transfection human melanoma lines
Transfection Human Melanoma Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pmc04571945-85-3-19?v=ATCC
Average 96 stars, based on 1 article reviews
transfection human melanoma lines - by Bioz Stars, 2026-07
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skmel28 cells  (Selleck Chemicals)
96
Selleck Chemicals skmel28 cells
Skmel28 Cells, supplied by Selleck Chemicals, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/bio_rxiv__2024__06__28__601235-40-2-10?v=Selleck+Chemicals
Average 96 stars, based on 1 article reviews
skmel28 cells - by Bioz Stars, 2026-07
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markers skmel 28  (ATCC)
94
ATCC markers skmel 28
Markers Skmel 28, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pm38790134-94-21-22?v=ATCC
Average 94 stars, based on 1 article reviews
markers skmel 28 - by Bioz Stars, 2026-07
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skmel 28  (ATCC)
94
ATCC skmel 28
Skmel 28, supplied by ATCC, used in various techniques. Bioz Stars score: 94/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pmc09244849-3-2-4?v=ATCC
Average 94 stars, based on 1 article reviews
skmel 28 - by Bioz Stars, 2026-07
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specimen 1 2a3 2a3 human crl 3212 hpv dna sequences cell 2 hepg2 hepg2 human hb 8065 cell 3 skmel 28 skmel 28  (ATCC)
99
ATCC specimen 1 2a3 2a3 human crl 3212 hpv dna sequences cell 2 hepg2 hepg2 human hb 8065 cell 3 skmel 28 skmel 28
Specimen 1 2a3 2a3 Human Crl 3212 Hpv Dna Sequences Cell 2 Hepg2 Hepg2 Human Hb 8065 Cell 3 Skmel 28 Skmel 28, supplied by ATCC, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pmc06882348__13014_2019_1418_MOESM2_ESM-8-47-43?v=ATCC
Average 99 stars, based on 1 article reviews
specimen 1 2a3 2a3 human crl 3212 hpv dna sequences cell 2 hepg2 hepg2 human hb 8065 cell 3 skmel 28 skmel 28 - by Bioz Stars, 2026-07
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cell culture human melanoma lines  (ATCC)
85
ATCC cell culture human melanoma lines
Cell Culture Human Melanoma Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 85/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pmc03422428-54-0-12?v=ATCC
Average 85 stars, based on 1 article reviews
cell culture human melanoma lines - by Bioz Stars, 2026-07
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emem  (Lonza)
90
Lonza emem
( a ) FBXW7 deficiency stabilizes nuclear HSF1 in melanoma. Nuclear fractions from wild type (COLO829, <t>SKMEL28,</t> SKMEL5, SKMEL24) and deficient for FBXW7 (WC00125, WM3862, WM39) melanoma cell lines were analyzed by immunoblotting as indicated. ( b ) FBXW7 knockdown results in nuclear HSF1 accumulation. 501mel cells were treated with non-coding (NC) siRNA or siRNA against FBXW7 . Nuclear fractions were analyzed by immunoblotting as indicated. ( c ) FBXW7 knockdown results in increased HSF1 targets expression in melanoma. DNAJB1 and HSP90AB1 mRNA expression in 501mel cells treated with non-coding (NC) siRNA or siRNA against FBXW7 ( P <0.001 for NC siRNA versus FBXW7 siRNA; unpaired t -test, and n=3 independent experiments) ( d ) 451Lu cells were treated with the BRAF inhibitor Vemurafenib (2 μM, 9 h) and MEK inhibitor Trametinib (50 nM, 9 h). Nuclear fractions were analyzed by immunoblotting as indicated. ( e ) FBXW7 depletion results in increased invasion in melanoma. 451Lu, SKMEL239 and A375 cells were infected with the indicated shRNA-encoding lentiviruses. One week after transduction, trans-well invasion assay was performed ( P <0.05 for 451Lu sh LUC versus sh FBXW7 , P <0.001 for SKMEL239 scrambled versus sh FBXW7 , P >0.05 for A375 sh LUC versus sh FBXW7 ; n= 10 fields per biological replicate; 4 biological replicates; unpaired t -test). Nuclear fractions were analyzed by immunoblotting as indicated. ( f ) Representative microphotographs of 451Lu and A375 cells after 8 days of infection with the indicated shRNA-encoding lentiviruses (scale bar, 200 μm). Error bars indicate mean ± SD.. Uncropped blots are shown in .
Emem, supplied by Lonza, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pmc04401662-194-16-19?v=Lonza
Average 90 stars, based on 1 article reviews
emem - by Bioz Stars, 2026-07
90/100 stars
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human melanoma skmel-28 cells  (BioResource International Inc)
90
BioResource International Inc human melanoma skmel-28 cells
( a ) FBXW7 deficiency stabilizes nuclear HSF1 in melanoma. Nuclear fractions from wild type (COLO829, <t>SKMEL28,</t> SKMEL5, SKMEL24) and deficient for FBXW7 (WC00125, WM3862, WM39) melanoma cell lines were analyzed by immunoblotting as indicated. ( b ) FBXW7 knockdown results in nuclear HSF1 accumulation. 501mel cells were treated with non-coding (NC) siRNA or siRNA against FBXW7 . Nuclear fractions were analyzed by immunoblotting as indicated. ( c ) FBXW7 knockdown results in increased HSF1 targets expression in melanoma. DNAJB1 and HSP90AB1 mRNA expression in 501mel cells treated with non-coding (NC) siRNA or siRNA against FBXW7 ( P <0.001 for NC siRNA versus FBXW7 siRNA; unpaired t -test, and n=3 independent experiments) ( d ) 451Lu cells were treated with the BRAF inhibitor Vemurafenib (2 μM, 9 h) and MEK inhibitor Trametinib (50 nM, 9 h). Nuclear fractions were analyzed by immunoblotting as indicated. ( e ) FBXW7 depletion results in increased invasion in melanoma. 451Lu, SKMEL239 and A375 cells were infected with the indicated shRNA-encoding lentiviruses. One week after transduction, trans-well invasion assay was performed ( P <0.05 for 451Lu sh LUC versus sh FBXW7 , P <0.001 for SKMEL239 scrambled versus sh FBXW7 , P >0.05 for A375 sh LUC versus sh FBXW7 ; n= 10 fields per biological replicate; 4 biological replicates; unpaired t -test). Nuclear fractions were analyzed by immunoblotting as indicated. ( f ) Representative microphotographs of 451Lu and A375 cells after 8 days of infection with the indicated shRNA-encoding lentiviruses (scale bar, 200 μm). Error bars indicate mean ± SD.. Uncropped blots are shown in .
Human Melanoma Skmel 28 Cells, supplied by BioResource International Inc, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pmc05288971-25-0-7?v=BioResource+International+Inc
Average 90 stars, based on 1 article reviews
human melanoma skmel-28 cells - by Bioz Stars, 2026-07
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human cancer cell lines  (ATCC)
96
ATCC human cancer cell lines
( a ) FBXW7 deficiency stabilizes nuclear HSF1 in melanoma. Nuclear fractions from wild type (COLO829, <t>SKMEL28,</t> SKMEL5, SKMEL24) and deficient for FBXW7 (WC00125, WM3862, WM39) melanoma cell lines were analyzed by immunoblotting as indicated. ( b ) FBXW7 knockdown results in nuclear HSF1 accumulation. 501mel cells were treated with non-coding (NC) siRNA or siRNA against FBXW7 . Nuclear fractions were analyzed by immunoblotting as indicated. ( c ) FBXW7 knockdown results in increased HSF1 targets expression in melanoma. DNAJB1 and HSP90AB1 mRNA expression in 501mel cells treated with non-coding (NC) siRNA or siRNA against FBXW7 ( P <0.001 for NC siRNA versus FBXW7 siRNA; unpaired t -test, and n=3 independent experiments) ( d ) 451Lu cells were treated with the BRAF inhibitor Vemurafenib (2 μM, 9 h) and MEK inhibitor Trametinib (50 nM, 9 h). Nuclear fractions were analyzed by immunoblotting as indicated. ( e ) FBXW7 depletion results in increased invasion in melanoma. 451Lu, SKMEL239 and A375 cells were infected with the indicated shRNA-encoding lentiviruses. One week after transduction, trans-well invasion assay was performed ( P <0.05 for 451Lu sh LUC versus sh FBXW7 , P <0.001 for SKMEL239 scrambled versus sh FBXW7 , P >0.05 for A375 sh LUC versus sh FBXW7 ; n= 10 fields per biological replicate; 4 biological replicates; unpaired t -test). Nuclear fractions were analyzed by immunoblotting as indicated. ( f ) Representative microphotographs of 451Lu and A375 cells after 8 days of infection with the indicated shRNA-encoding lentiviruses (scale bar, 200 μm). Error bars indicate mean ± SD.. Uncropped blots are shown in .
Human Cancer Cell Lines, supplied by ATCC, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/wild+type+wt+skmel28+cells/pmc05830651-43-1-27?v=ATCC
Average 96 stars, based on 1 article reviews
human cancer cell lines - by Bioz Stars, 2026-07
96/100 stars
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Image Search Results


( a ) FBXW7 deficiency stabilizes nuclear HSF1 in melanoma. Nuclear fractions from wild type (COLO829, SKMEL28, SKMEL5, SKMEL24) and deficient for FBXW7 (WC00125, WM3862, WM39) melanoma cell lines were analyzed by immunoblotting as indicated. ( b ) FBXW7 knockdown results in nuclear HSF1 accumulation. 501mel cells were treated with non-coding (NC) siRNA or siRNA against FBXW7 . Nuclear fractions were analyzed by immunoblotting as indicated. ( c ) FBXW7 knockdown results in increased HSF1 targets expression in melanoma. DNAJB1 and HSP90AB1 mRNA expression in 501mel cells treated with non-coding (NC) siRNA or siRNA against FBXW7 ( P <0.001 for NC siRNA versus FBXW7 siRNA; unpaired t -test, and n=3 independent experiments) ( d ) 451Lu cells were treated with the BRAF inhibitor Vemurafenib (2 μM, 9 h) and MEK inhibitor Trametinib (50 nM, 9 h). Nuclear fractions were analyzed by immunoblotting as indicated. ( e ) FBXW7 depletion results in increased invasion in melanoma. 451Lu, SKMEL239 and A375 cells were infected with the indicated shRNA-encoding lentiviruses. One week after transduction, trans-well invasion assay was performed ( P <0.05 for 451Lu sh LUC versus sh FBXW7 , P <0.001 for SKMEL239 scrambled versus sh FBXW7 , P >0.05 for A375 sh LUC versus sh FBXW7 ; n= 10 fields per biological replicate; 4 biological replicates; unpaired t -test). Nuclear fractions were analyzed by immunoblotting as indicated. ( f ) Representative microphotographs of 451Lu and A375 cells after 8 days of infection with the indicated shRNA-encoding lentiviruses (scale bar, 200 μm). Error bars indicate mean ± SD.. Uncropped blots are shown in .

Journal: Nature cell biology

Article Title: FBXW7 modulates cellular stress response and metastatic potential via HSF1 post-translational modification

doi: 10.1038/ncb3121

Figure Lengend Snippet: ( a ) FBXW7 deficiency stabilizes nuclear HSF1 in melanoma. Nuclear fractions from wild type (COLO829, SKMEL28, SKMEL5, SKMEL24) and deficient for FBXW7 (WC00125, WM3862, WM39) melanoma cell lines were analyzed by immunoblotting as indicated. ( b ) FBXW7 knockdown results in nuclear HSF1 accumulation. 501mel cells were treated with non-coding (NC) siRNA or siRNA against FBXW7 . Nuclear fractions were analyzed by immunoblotting as indicated. ( c ) FBXW7 knockdown results in increased HSF1 targets expression in melanoma. DNAJB1 and HSP90AB1 mRNA expression in 501mel cells treated with non-coding (NC) siRNA or siRNA against FBXW7 ( P <0.001 for NC siRNA versus FBXW7 siRNA; unpaired t -test, and n=3 independent experiments) ( d ) 451Lu cells were treated with the BRAF inhibitor Vemurafenib (2 μM, 9 h) and MEK inhibitor Trametinib (50 nM, 9 h). Nuclear fractions were analyzed by immunoblotting as indicated. ( e ) FBXW7 depletion results in increased invasion in melanoma. 451Lu, SKMEL239 and A375 cells were infected with the indicated shRNA-encoding lentiviruses. One week after transduction, trans-well invasion assay was performed ( P <0.05 for 451Lu sh LUC versus sh FBXW7 , P <0.001 for SKMEL239 scrambled versus sh FBXW7 , P >0.05 for A375 sh LUC versus sh FBXW7 ; n= 10 fields per biological replicate; 4 biological replicates; unpaired t -test). Nuclear fractions were analyzed by immunoblotting as indicated. ( f ) Representative microphotographs of 451Lu and A375 cells after 8 days of infection with the indicated shRNA-encoding lentiviruses (scale bar, 200 μm). Error bars indicate mean ± SD.. Uncropped blots are shown in .

Article Snippet: 501mel, MM485 and Colo829 were maintained in RPMI 1640 (Gibco) containing 10% (v/v) FBS; SKMEL5 and SKMEL28 in EMEM (Lonza) containing 10% (v/v) FBS; SKMEL24 in MEM (Lonza) containing 10% (v/v) FBS; WC00125, WM3862 and WM39 in tumor media (MCDB153 containing 20% Leibovitz's L-15, 2% fetal bovine serum, 5 μg/ml insulin, 1.68 mM CaCl 2 ).

Techniques: Western Blot, Expressing, Infection, shRNA, Transduction, Invasion Assay